About the Topic

Ultra-high-pressure liquid chromatography (UHPLC) is the new standard HPLC platform delivering higher performance with small-particle columns used on low-dispersion systems with pressure limits of 15,000 – 23,000 psi.  This intermediate-level 1/2-day training workshop will provide a critical overview the fundamental concepts, benefits, potential issues, and best practices of UHPLC in pharmaceutical analysis of small molecule drugs and recombinant biologics (monoclonal antibodies, mAbs).

 Who Should Attend

This workshop is intended for analysts, managers, and researchers wishing to have a thorough understanding the fundamentals and practices of UHPLC in pharmaceutical analysis. A basic knowledge of HPLC is assumed and some practical hands-on experience is highly recommended.

Workshop Agenda

UHPLC 1: Concept and Benefits  

1. Overview and perspectives, Glossary, the modern HPLC platform with higher performance, UHPLC concepts (the need for high pressure, void volume, peak volume, instrumental bandwidth), UV flow cell design, dwell volume.
2. Benefits and case studies: Very fast separations with good resolution, very high resolution analysis of complex samples, facilitating rapid method development, flexibility to customize resolution, a modernized generic method for most drugs, a greener HPLC platform. 

UHPLC 2:  Potential Issues, Practices,  and implementation  

1. Potential issues and how to mitigate (viscous heating, operating nuances, compatibility to existing methods, injection precision, detector sensitivity vs. mixing volumes.
2. Method translation (transfer) issues: method portability, method conversion (translation), “What is method transfer?”, need for partial method validation, case studies.
3. How to transition from HPLC to UHPLC, end-fittings, system performance tradeoffs, Myths in UHPLC, references.

Learning Outcomes UHPLC in Biopharmaceutical Analysis

The attendee will learn the benefits, best practices and potential issues of UHPLC in pharmaceutical analysis including method translation and how to get started in UHPLC.