Method development for the chromatographic separation of biomolecules can be a daunting task when you consider all the options available to you. How do you choose the right chromatographic mode, the right mobile phase, and the right column? This presentation will give you some guidelines to help you make these choices as well as some valuable tips to develop robust methods. The focus will be on Size Exclusion, Reverse Phase, and Ion Exchange Chromatography of  biomolecules.

The presentation will review the mechanisms of these separation techniques and show how to optimize separation conditions for the best resolution in the least amount of time with HPLC, UHPLC and LC/MS instrumentation.